Anti-tPA (human, tissue plasminogen activator)
Mouse monoclonal antibody
Cat.No. MON T-1
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Tissue plasminogen activator (tPA) is a serine protease which converts the abundant proenzyme plasminogen to active plasmin. It plays a key role in thrombolysis (1) and several neurobiological processes (2).
Native human tPA
MON T-1 is specific for human tPA. No reaction is seen with human urokinase plasminogen activator (uPA) when tested by ELISA, immunoblotting and enzyme inhibition and no reaction with any other human plasma proteins is seen when tested by immunoblotting.
MON T-1 binds to the A-chain of tPA (3)
MON T-1 reacts with single- and two-chain tPA in ELISA, Western blotting and immunohistochemistry, and can also be used in immunopurification. It inhibits the enzymatic activity of tPA. For immunohistochemistry applications MON T-1 can be used on frozen sections.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 4 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Yes | 5,6 |
1. Collen D, Lijnen HR (2005) Thrombolytic agents. Thromb Haemost 93:627-630.
2. Melchior JP, Strickland S (2005) Tissue plasminogen activator in central nervous system physiology and pathology. Thromb Haemost 93:655-60.
3. Nielsen LS, Hansen JG, Andreasen PA, Skriver L, Danø K, Zeuthen J (1983) Monoclonal antibody to human 66 000 molecular weight plasminogen activator from melanoma cells. Specific enzyme inhibition and one step affinity purification. EMBO J 2:115-119.
4. Lund LR, Georg B, Nielsen LS, Mayer M, Danø K, Andreasen PA (1988) Plasminogen activator inhibitor type 1: Cell specific and differentiation-induced expression and regulation in human cell lines, as determined by enzyme linked immunosorbent assay. Mol Cell Endocrinol 60:43-53.
5. Kristensen P, Larsson LI, Nielsen LS, Grøndahl-Hansen J, Andreasen PA, Danø K (1984) Human endothelial cells contain one type of plasminogen activator. FEBS Lett 168:33-37.
6. Grøndahl-Hansen J, Ralfkiær E, Nielsen LS, Kristensen P, Frentz G, Danø K (1987) Immunohistochemical localization of urokinase - and tissue-type plasminogen activators in psoriatic skin. J Invest Dermatol 88:28-32.
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